The scientific productivity of the B3OA laboratory includes research and clinical publications and is characterized by (i) an increase in the total number of publications from 236 in 2007-2012 to 371 in 2012-2017 and (ii) an increase in the number of publications considered exceptional (from 37 in 2007-2012 to 44 in 2012-2017) and excellent (from 57 in 2007-2012 to 112 in 2007-2017) by INIST CNRS.

Our latest publications

Enzyme-controlled, nutritive hydrogel for mesenchymal stromal cell survival and paracrine functions

We hereby propose a paradigm shift for enhancing MSC survival by designing, developing, and testing an enzyme-controlled, nutritive hydrogel with an inbuilt glucose delivery system.

Glucose depletion decreases cell viability without triggering degenerative changes in a physiological nucleus pulposus explant model

The present study provided evidence for glucose depletion as an essential player in NP cell viability but also suggested that other microenvironment factor(s) may be involved in triggering the typical shift of NP cell phenotype observed during disc degeneration.

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells

These results show that the physico-chemical properties of collagen/THA composite hydrogels and GDF5/TGFβ1 act in synergy to promote the differentiation of BM-MSCs into NP like cells.

3D-Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical-Size Bone Defect

A new osteoinductive medical device is developed here for long bone repair by combining a 3D-printed architectured cylindrical scaffold made of clinical-grade polylactic acid (PLA) with a polyelectrolyte film coating delivering the osteogenic bone morphogenetic protein 2 (BMP-2).

The Lower in Vivo Osteogenicity of Adipose Tissue-Derived Stem Cells Correlates with a Higher Innate Immune Response

These results proved that ATSCs are a transient source of inflammatory cytokines promoting a transient immune response post-implantation; this milieu correlates with impaired osteogenic differentiation of both the implanted ATSCs and the host osteoprogenitor cells.

A New Microarchitecture-Based Parameter to Predict the Micromechanical Properties of Bone Allografts

This study presents a novel microarchitectural parameter that could be used to predict the level of mechanical stimulation at the cellular level, a key parameter to better understand and optimize the mechanism of osteogenesis in bone tissue engineering.

Three-dimensional Printing of Biomimetic Titanium Mimicking Trabecular Bone Induces Human Mesenchymal Stem Cell Proliferation: An In-vitro Analysis

The aim of this study was to assess the effect of three-dimensional (3D) printing of porous titanium on human mesenchymal stem cell (hMSC) adhesion, proliferation, and osteogenic differentiation.

Glucose Metabolism: Optimizing Regenerative Functionalities of Mesenchymal Stromal Cells Postimplantation

Following a brief overview of glucose metabolism and regulation in MSCs, the present literature review includes recent pertinent findings that challenge old paradigms and notions

Mitohormesis and mitochondrial dynamics in the regulation of stem cell fate

This review aimed to provide an update to a broader concept of stem cell fate in stress conditions of damaged tissues, and insights for the mitochondrial hormesis (mitohormesis), including the integrated stress response (ISR), mitochondrial dynamics, mitochondria uncoupling, unfolded protein response, and mitokines, with implications for the control of stem…

In Vivo bone tissue induction by freeze-dried collagen-nanohydroxyapatite matrix loaded with BMP2/NS1 mRNAs lipopolyplexes

Overall, our results demonstrate that the BMP2/NS1 dual mRNAs system is suitable for osteogenic engagement, and the freeze-dried RAM-BMP2/NS1 could be promising off-the-shelf products for clinical orthopedic practice

Virtual monoenergetic images from photon-counting spectral computed tomography to assess knee osteoarthritis

Virtual monoenergetic images may provide direct visualisation of both cartilage and bone details. Thus, unenhanced PCSCT appears to be a new modality for characterising the knee joint with the potential to increase the diagnostic capability of computed tomography for joint diseases and osteoarthritis.

Experimental Type 2 Diabetes Differently Impacts on the Select Functions of Bone Marrow-Derived Multipotent Stromal Cells

These results provided evidence that the type 2 diabetes environment impairs multipotent stromal cell expansion and select functions pertinent to their efficacy when used in autologous cell therapies.

Human dentin characteristics of patients with osteogenesis imperfecta: insights into collagen-based biomaterials

We investigated the collagen, mineral and mechanical properties of dentin from deciduous teeth collected from patients with mild form of osteogenesis imperfecta and displaying no obvious clinical signs of dentinogenesis imperfecta. For the first time, an increase in the hardness of osteogenesis imperfecta dentin associated with an increase in mineral…

Does physical exercise always improve bone quality in rats?

This literature review presents for the first time the effects on five exercise models (treadmill running, wheel running, swimming, resistance training and vibration modes) in three different experimental rat groups (males, females, osteopenic) on bone quality.

Does eccentric-concentric resistance training improve early functional outcomes compared to concentric resistance training after total knee arthroplasty?

This study showed that early combined eccentric-concentric rehabilitation after total knee arthroplasty appears to be associated with improved outcomes compared to classic concentric rehabilitation protocols, on both physical function and quality of life.

Osteogenic-differentiated mesenchymal stem cell-secreted extracellular matrix as a bone morphogenetic protein-2 delivery system for ectopic bone formation

This study provided evidence that the osteoinductive potential of BMP-2 was greater when loaded within an osteogenic MSC-derived ECM.

Impact of astaxanthin on diabetes pathogenesis and chronic complications

In the present review, we aimed to identify the up-to-date biological effects and underlying mechanisms of astaxanthin on the ROS-induced diabetes mellitus-associated metabolic disorders and subsequent complications

Non invasive process for the evaluation of the quality of internal dense connective tissues

The invention relates to a non-invasive process for evaluating the quality of one or more dense connective tissue(s) in a patient.

Custom-made macroporous bioceramic implants based on triply-periodic minimal surfaces for bone defects in load-bearing sites

This study showed, for the first time, that custom-made TPMS-based bioceramic implants could be produced and successfully implanted in load-bearing sites

Concise review: Understanding and leveraging cell metabolism to enhance mesenchymal stem cell transplantation survival in tissue engineering and regenerative medicine applications

Here, we provide a comprehensive review of plausible metabolic switches in response to implantation and of the various strategies currently used to leverage MSC metabolism to improve stem cell-based therapeutics.