We hereby propose a paradigm shift for enhancing MSC survival by designing, developing, and testing an enzyme-controlled, nutritive hydrogel with an inbuilt glucose delivery system.
The present study provided evidence for glucose depletion as an essential player in NP cell viability but also suggested that other microenvironment factor(s) may be involved in triggering the typical shift of NP cell phenotype observed during disc degeneration.
These results show that the physico-chemical properties of collagen/THA composite hydrogels and GDF5/TGFβ1 act in synergy to promote the differentiation of BM-MSCs into NP like cells.
A new osteoinductive medical device is developed here for long bone repair by combining a 3D-printed architectured cylindrical scaffold made of clinical-grade polylactic acid (PLA) with a polyelectrolyte film coating delivering the osteogenic bone morphogenetic protein 2 (BMP-2).
These results proved that ATSCs are a transient source of inflammatory cytokines promoting a transient immune response post-implantation; this milieu correlates with impaired osteogenic differentiation of both the implanted ATSCs and the host osteoprogenitor cells.
This study presents a novel microarchitectural parameter that could be used to predict the level of mechanical stimulation at the cellular level, a key parameter to better understand and optimize the mechanism of osteogenesis in bone tissue engineering.
The aim of this study was to assess the effect of three-dimensional (3D) printing of porous titanium on human mesenchymal stem cell (hMSC) adhesion, proliferation, and osteogenic differentiation.
Following a brief overview of glucose metabolism and regulation in MSCs, the present literature review includes recent pertinent findings that challenge old paradigms and notions
This review aimed to provide an update to a broader concept of stem cell fate in stress conditions of damaged tissues, and insights for the mitochondrial hormesis (mitohormesis), including the integrated stress response (ISR), mitochondrial dynamics, mitochondria uncoupling, unfolded protein response, and mitokines, with implications for the control of stem cells programing in a successful clinical cell therapy
Overall, our results demonstrate that the BMP2/NS1 dual mRNAs system is suitable for osteogenic engagement, and the freeze-dried RAM-BMP2/NS1 could be promising off-the-shelf products for clinical orthopedic practice
Virtual monoenergetic images may provide direct visualisation of both cartilage and bone details. Thus, unenhanced PCSCT appears to be a new modality for characterising the knee joint with the potential to increase the diagnostic capability of computed tomography for joint diseases and osteoarthritis.
These results provided evidence that the type 2 diabetes environment impairs multipotent stromal cell expansion and select functions pertinent to their efficacy when used in autologous cell therapies.
We investigated the collagen, mineral and mechanical properties of dentin from deciduous teeth collected from patients with mild form of osteogenesis imperfecta and displaying no obvious clinical signs of dentinogenesis imperfecta. For the first time, an increase in the hardness of osteogenesis imperfecta dentin associated with an increase in mineral content compared to healthy patients was reported.
This literature review presents for the first time the effects on five exercise models (treadmill running, wheel running, swimming, resistance training and vibration modes) in three different experimental rat groups (males, females, osteopenic) on bone quality.
This study showed that early combined eccentric-concentric rehabilitation after total knee arthroplasty appears to be associated with improved outcomes compared to classic concentric rehabilitation protocols, on both physical function and quality of life.
This study provided evidence that the osteoinductive potential of BMP-2 was greater when loaded within an osteogenic MSC-derived ECM.
In the present review, we aimed to identify the up-to-date biological effects and underlying mechanisms of astaxanthin on the ROS-induced diabetes mellitus-associated metabolic disorders and subsequent complications
This study showed, for the first time, that custom-made TPMS-based bioceramic implants could be produced and successfully implanted in load-bearing sites
Here, we provide a comprehensive review of plausible metabolic switches in response to
implantation and of the various strategies currently used to leverage MSC metabolism
to improve stem cell-based therapeutics.
Our results showed that the addition of only low dose of BMP-2 was beneficial to enhance the bone-forming potential of MSCs, whereas high dose of BMP-2 overcame the advantage of combining this growth factor with MSCs.
In this uncontrolled study, airway bioengineering using stented aortic matrices demonstrated feasibility for complex tracheal and bronchial reconstruction.
The present study provided evidence that T2DM impairs bone formation in critical-size calvarial defects and markedly reduces angiogenesis in all defects regardless of the defect size tested.